Responsive hydrogel binding matrix for dual signal amplification in fluorescence affinity biosensors and peptide microarrays
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F21%3A00552381" target="_blank" >RIV/68378271:_____/21:00552381 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0327527" target="_blank" >http://hdl.handle.net/11104/0327527</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsami.1c05950" target="_blank" >10.1021/acsami.1c05950</a>
Alternative languages
Result language
angličtina
Original language name
Responsive hydrogel binding matrix for dual signal amplification in fluorescence affinity biosensors and peptide microarrays
Original language description
A combined approach to signal enhancement in fluorescence affinity biosensors and assays is reported. It is based on the compaction of specifically captured target molecules at the sensor surface followed by optical probing with a tightly confined surface plasmon (SP) field. This concept is utilized by using a thermoresponsive hydrogel(HG) binding matrix that is prepared from a terpolymer derived from poly(N-isopropylacrylamide) (pNIPAAm) and attached to a metallic sensor surface. Epi-illumination fluorescence and SP-enhanced total internal reflection fluorescence readouts of affinity binding events are performed to spatially interrogate the fluorescent signal in the direction parallel and perpendicular to the sensor surface. The pNIPAAm-based HG binding matrix is arranged in arrays of sensing spots and employed for the specific detection of human IgG antibodies against the Epstein−Barr virus (EBV).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
<a href="/en/project/EF18_053%2F0016627" target="_blank" >EF18_053/0016627: FZU researchers, technical and administrative staff mobility</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Applied Materials and Interfaces
ISSN
1944-8244
e-ISSN
1944-8252
Volume of the periodical
13
Issue of the periodical within the volume
23
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
27645-27655
UT code for WoS article
000664289800096
EID of the result in the Scopus database
2-s2.0-85108386869