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Scalable production of tissue-like vascularized liver organoids from human PSCs

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F23%3A00576064" target="_blank" >RIV/68378271:_____/23:00576064 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11150/23:10469520

  • Result on the web

    <a href="https://hdl.handle.net/11104/0346848" target="_blank" >https://hdl.handle.net/11104/0346848</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/s12276-023-01074-1" target="_blank" >10.1038/s12276-023-01074-1</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Scalable production of tissue-like vascularized liver organoids from human PSCs

  • Original language description

    The lack of physiological parity between 2D cell culture and in vivo culture has led to the development of more organotypic models, such as organoids. Organoid models have been developed for a number of tissues, including the liver. Current organoid protocols are characterized by a reliance on extracellular matrices (ECMs), patterning in 2D culture, costly growth factors and a lack of cellular diversity, structure, and organization. Current hepatic organoid models are generally simplistic and composed of hepatocytes or cholangiocytes. We have developed an approach that does not require 2D patterning, is ECM independent, and employs small molecules to mimic embryonic liver development that produces large quantities of liver-like organoids. The organoids exhibit key liver functions, including drug metabolism, serum protein production, urea synthesis and coagulation factor production, with preserved post-translational modifications such as N-glycosylation and functionality.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10610 - Biophysics

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Experimental and Molecular Medicine

  • ISSN

    1226-3613

  • e-ISSN

    2092-6413

  • Volume of the periodical

    55

  • Issue of the periodical within the volume

    9

  • Country of publishing house

    KR - KOREA, REPUBLIC OF

  • Number of pages

    20

  • Pages from-to

    2005-2024

  • UT code for WoS article

    001065142700006

  • EID of the result in the Scopus database

    2-s2.0-85169166859