Macroporous Hydrogels for Cell Cultivations and Their 3-D Visualization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F19%3A00339038" target="_blank" >RIV/68407700:21220/19:00339038 - isvavai.cz</a>

Result on the web

<a href="http://mech.fs.cvut.cz" target="_blank" >http://mech.fs.cvut.cz</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Macroporous Hydrogels for Cell Cultivations and Their 3-D Visualization

Original language description

Macromolecular hydrogels provide model synthetic substrates widely applied in biomedical research, especially for studies of the cellular settlement. Today, hydrogels are tailored with respect to specific needs of the given model or a real tissue. Key characteristics of the hydrogel substrates are their chemical composition, morphological structure, and, most notably, the porosity and its topology: size, shape, and mutual connectivity of pores. To develop new substrates, it is therefore necessary to use methods for the visualization and reconstruction of their inner structure in space and to characterize, if possible quantify, its parameters, such as the total volume of the pores, the fraction of communicating pores, characteristic patency and the total area of the inner walls of the connecting corridors. Fluorescent confocal microscopy in combination with an advanced software represents an efficient method to determine parameters mentioned above, in order to characterize hydrogels in situ, i.e., in the state of equilibrium swelling in water. Thus, this method differs significantly from another widespread technique, i.e., the scanning electron microscopy, where the samples, saturated with water, have to be freeze-dried first, and then the structure of each sample is determined in the frozen state. This paper compares the results of both imaging methods, as applied to macroporous hydrogels. Advantages and shortcuts of both methods are discussed. English translation available in the on-line version

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20401 - Chemical engineering (plants, products)

Project

<a href="/en/project/GA17-08531S" target="_blank" >GA17-08531S: Computational design of hydrogel cell scaffolds.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemické listy

ISSN

0009-2770

e-ISSN

1213-7103

Volume of the periodical

113

Issue of the periodical within the volume

12

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

726-731

UT code for WoS article

000502333500009

EID of the result in the Scopus database

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