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Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F19%3A00332285" target="_blank" >RIV/68407700:21230/19:00332285 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.1038/s41592-019-0364-4" target="_blank" >https://doi.org/10.1038/s41592-019-0364-4</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/s41592-019-0364-4" target="_blank" >10.1038/s41592-019-0364-4</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

  • Original language description

    With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule localization microscopy (SMLM), a large set of different data analysis packages have been developed to generate super-resolution images. In a large community effort, we designed a competition to extensively characterize and rank the performance of 2D and 3D SMLM software packages. We generated realistic simulated datasets for popular imaging modalities—2D, astigmatic 3D, biplane 3D and double-helix 3D—and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D SMLM software and provides a holistic view of how the latest 2D and 3D SMLM packages perform in realistic conditions. This resource allows researchers to identify optimal analytical software for their experiments, allows 3D SMLM software developers to benchmark new software against the current state of the art, and provides insight into the current limits of the field.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10609 - Biochemical research methods

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Nature Methods

  • ISSN

    1548-7091

  • e-ISSN

    1548-7105

  • Volume of the periodical

    16

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    9

  • Pages from-to

    387-395

  • UT code for WoS article

    000466430000019

  • EID of the result in the Scopus database

    2-s2.0-85064068699