Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F19%3A00332285" target="_blank" >RIV/68407700:21230/19:00332285 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1038/s41592-019-0364-4" target="_blank" >https://doi.org/10.1038/s41592-019-0364-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41592-019-0364-4" target="_blank" >10.1038/s41592-019-0364-4</a>
Alternative languages
Result language
angličtina
Original language name
Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software
Original language description
With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule localization microscopy (SMLM), a large set of different data analysis packages have been developed to generate super-resolution images. In a large community effort, we designed a competition to extensively characterize and rank the performance of 2D and 3D SMLM software packages. We generated realistic simulated datasets for popular imaging modalities—2D, astigmatic 3D, biplane 3D and double-helix 3D—and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D SMLM software and provides a holistic view of how the latest 2D and 3D SMLM packages perform in realistic conditions. This resource allows researchers to identify optimal analytical software for their experiments, allows 3D SMLM software developers to benchmark new software against the current state of the art, and provides insight into the current limits of the field.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Methods
ISSN
1548-7091
e-ISSN
1548-7105
Volume of the periodical
16
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
387-395
UT code for WoS article
000466430000019
EID of the result in the Scopus database
2-s2.0-85064068699