New luminescence lifetime macro-imager based on a Tpx3Cam optical camera
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F20%3A00336514" target="_blank" >RIV/68407700:21340/20:00336514 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1364/BOE.11.000077" target="_blank" >https://doi.org/10.1364/BOE.11.000077</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1364/BOE.11.000077" target="_blank" >10.1364/BOE.11.000077</a>
Alternative languages
Result language
angličtina
Original language name
New luminescence lifetime macro-imager based on a Tpx3Cam optical camera
Original language description
The properties of a novel ultra-fast optical imager, Tpx3Cam, were investigated for macroscopic wide-field phosphorescent lifetime imaging (PLIM) applications. The camera is based on a novel optical sensor and Timepix3 readout chip with a time resolution of 1.6 ns, recording of photon arrival time and time over threshold for each pixel, and readout rate of 80 megapixels per second. In this study, we coupled the camera to an image intensifier, a 760 nm emission filter and a 50 mm lens, and with a super-bright 627nm LED providing pulsed excitation of a 18 x 18 mm sample area. The resulting macro-imager with compact and rigid optical alignment of its main components was characterised using planar phosphorescent O2 sensors and a resolution plate mask. Several acquisition and image processing algorithms were evaluated to optimise the system resolution and performance for the wide-field PLIM, followed by imaging a variety of phosphorescent samples. The new PLIM system looks promising, particularly for phosphorescence lifetime-based imaging of O2 in various chemical and biological samples.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomedical Optics Express
ISSN
2156-7085
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
77-88
UT code for WoS article
000519061700007
EID of the result in the Scopus database
2-s2.0-85078893842