Metal Ion Exchange in Zn-Dependent S1 Nuclease: Effect on the Structure

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F22%3A00360993" target="_blank" >RIV/68407700:21340/22:00360993 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Metal Ion Exchange in Zn-Dependent S1 Nuclease: Effect on the Structure

Original language description

S1 nuclease is an enzyme commonly used in biotechnology applications. Its activity depends on the presence of three Zn2+ ions in the active site. In this work, we examine the possibility of exchanging natively present Zn2+ ions with Cd2+. EDTA-treated S1 nuclease was successfully crystallised in the presence of CdCl2. The anomalous signal from three different energies confirmed the exchange of two Zn2+ ions to Cd2+. The residues of the active site remained structurally conserved.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10302 - Condensed matter physics (including formerly solid state physics, supercond.)

Project

<a href="/en/project/EF16_019%2F0000778" target="_blank" >EF16_019/0000778: Center for advanced applied science</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník příspěvků 11. studentské vědecké konference fyziky pevných látek a materiálů

ISBN

978-80-01-07079-6

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

57-61

Publisher name

katedra inženýrství pevných látek

Place of publication

Praha

Event location

Praha, FJFI

Event date

Sep 12, 2022

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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