Chitinolytic bacterial populations in gastrointestinal tract of wild ruminants

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F12%3A00202761" target="_blank" >RIV/68407700:21460/12:00202761 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Chitinolytic bacterial populations in gastrointestinal tract of wild ruminants
Original language description
In this study we focused on bacteria from the animal digestive tract with an ability to utilize chitin. As cultivation media we used M-10 medium with colloid chitin as source of energy. Cultivation was carried out under anaerobic conditions. Chitinolyticbacteria were isolated from enrichment culture with faeces of different wild ruminants (fallow deer, Pere David's deer, Roth schild's giraffe). Isolates were selected accoring to their activity towards CM chitin and then characterized. The identification of strains with chitinolytic activities was made due to sequencing method. For further characterization of biochemical ability to degrade chitin substrate we will perform polyacrylamide gel electrophoresis of proteins, which are produced by chitosan degradation. The visualization of the chitinolytic enzymes will be achieved by zymography technique.
Czech name
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Czech description
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Project
<a href="/en/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: BIO-XUV Research Team Advancement at FBME CTU</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Instuments and Methods for Biology and Medicine 2012
ISBN
978-80-01-05119-1
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
42-45
Publisher name
Czech Technical University
Place of publication
Prague
Event location
Kladno
Event date
May 29, 2012
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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