Platelet Lysate as a Serum Replacement for Skin Cell Culture on Biomimetic PCL Nanofibers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00317978" target="_blank" >RIV/68407700:21460/18:00317978 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21720/18:00317978 RIV/00216208:11130/18:10375268 RIV/00216208:11310/18:10375268 RIV/68378041:_____/18:00477248 RIV/62157124:16170/18:43878394
Result on the web
<a href="http://dx.doi.org/10.1080/09537104.2017.1316838" target="_blank" >http://dx.doi.org/10.1080/09537104.2017.1316838</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/09537104.2017.1316838" target="_blank" >10.1080/09537104.2017.1316838</a>

Result language
angličtina
Original language name
Platelet Lysate as a Serum Replacement for Skin Cell Culture on Biomimetic PCL Nanofibers
Original language description
Platelets are a popular source of native growth factors for tissue engineering applications. The aim of the study was to verify the use of platelet lysate as a fetal bovine serum (FBS) replacement for skin cell culture. The cytokine content of the platelet lysate was characterized using the Bio-Plex system. The cells (fibroblasts, melanocytes, and keratinocytes) were cultured on PCL nanofibrous scaffolds to mimic their natural microenvironment. The cytokine content of the platelet lysate was determined, and to the cells, a medium containing platelet lysate or platelet lysate in combination with FBS was added. The results showed that 7% (v/v) platelet lysate was sufficient to supplement 10% (v/v) FBS in the culture of fibroblasts and keratinocytes. The combination of platelet lysate and FBS had a rather inhibitory effect on fibroblasts, in contrary to keratinocytes, where the effect was synergic. Platelet lysate did not sufficiently promote proliferation in melanocytes; however, the combination of FBS and platelet lysate yielded a better outcome and resulted in bipolar morphology of the cultured melanocytes. The data indicated that platelet lysate improved cell proliferation and metabolic activity and may be used as an additive to the cell culture media.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30500 - Other medical sciences

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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