Three-dimensional interferometric scattering microscopy via remote focusing technique

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F20%3A00341425" target="_blank" >RIV/68407700:21460/20:00341425 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1364/OL.386172" target="_blank" >https://doi.org/10.1364/OL.386172</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1364/OL.386172" target="_blank" >10.1364/OL.386172</a>

Result language
angličtina
Original language name
Three-dimensional interferometric scattering microscopy via remote focusing technique
Original language description
Interferometric scattering (iSCAT) microscopy enables us to track nm-sized objects with high spatial and temporal resolutions and permits label-free imaging of biomolecules. Its superb sensitivity, however, comes at a cost by several downsides, such as slow three-dimensional imaging and limited vertical tracking. Here, we propose a new method, Remote Focusing-iSCAT (RF-iSCAT) microscopy, to visu- alize a volume specimen by imaging sections at different depths without translation of either the objective lens or sample stage. We demonstrate the principle of RF-iSCAT by determining the z-position of submicrometer beads by translating the reference mirror instead. RF-iSCAT features an unprecedentedly long range of vertical tracking and permits fast but vibration-free vertical scanning. We antici- pate that RF-iSCAT would enhance the utility of iSCAT for dynamics study.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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