Molecular genetics methods in use

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F20%3A00346181" target="_blank" >RIV/68407700:21460/20:00346181 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Molecular genetics methods in use
Original language description
The importance of studying microbial populations of the digestive tract in connection with various diseases is gaining relevance. Knowledge of the effect of leishmaniasis on the microbiome is limited. Genetically different individuals infected with the same species of parasite show a different composition of microbial populations of the digestive tract, which points to the importance of studying intestinal microbiota in relation to the host genotype. Aim of this study is a comparison of the composition of microbial populations of the digestive tract in health and disease depending on the host genotype. In the experimental model, we were able to define the effect of genetic background using several inbred strains in the chronic phase of infection and thus determine the effect of the disease itself. This work presents the molecular biology methods used for detailed analysis of immunopathological manifestations of Leishmania infection (PCR-ELISA) in combination with study of gut microbiome diversity (DGGE, NGS) in one experiment. These molecular genetics methods gave us a tool to prepare a more complex model of disease effects.
Czech name
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Czech description
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Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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