Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F21%3A00348912" target="_blank" >RIV/68407700:21460/21:00348912 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/21:10429930

Result on the web

<a href="https://doi.org/10.3390/ijms22041843" target="_blank" >https://doi.org/10.3390/ijms22041843</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms22041843" target="_blank" >10.3390/ijms22041843</a>

Result language

angličtina

Original language name

Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

Original language description

Vascular endothelial growth factor-A(165) (VEGF-A(165)) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A(165) and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A(165) stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A(165) on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A(165) have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/NV18-02-00422" target="_blank" >NV18-02-00422: New materials for cardiovascular surgery based on modified decellularized tissues</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1661-6596

e-ISSN

1422-0067

Volume of the periodical

22

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

26

Pages from-to

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UT code for WoS article

000623786800001

EID of the result in the Scopus database

2-s2.0-85100662467