LABORATORY WATER-WINDOW MICROSCOPE BASED ON Z-PINCHING CAPILLARY DISCHARGE SOURCE FOR BIOLOGICAL IMAGING

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F21%3A00353244" target="_blank" >RIV/68407700:21460/21:00353244 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

LABORATORY WATER-WINDOW MICROSCOPE BASED ON Z-PINCHING CAPILLARY DISCHARGE SOURCE FOR BIOLOGICAL IMAGING

Original language description

Soft X-ray (SXR) microscopy in the water-window spectral region (2.28-4.36 nm) is one of the emerging biological imaging methods with the resolution in the order of tens nanometers covering the gap between transmission el. microscopy and visible light microscopy, and providing a tool to image an intact cell in a native or near-native environment. However, SXR microscopes are commonly based on large-scale facilities such as synchrotron or free-electron lasers, limiting the accessibility to this imaging technique for the broader scientific community. In the first section this Thesis describes comparison of two table-top sources of soft X-ray radiation -- laser- and discharge-produced plasma, with regards to imaging in the water-window region. The laser-produced plasma source was developed in LLG, Germany, and plasma is induced by a laser pulse of Q-switched Nd:YAG laser focused into nitrogen gas-puff target. The discharge-produced plasma source was developed in-house at CTU in Prague, and plasma is generated by a current discharge through the ceramic capillary filled with nitrogen gas. Both sources utilize the nitrogen spectral line 2.88 nm (430 eV). The discharge-produced nitrogen plasma source has a higher brightness; therefore, it was used for the microscope development. In its second section the Thesis presents a design and performance of a compact transmission water-window microscope based on the Z-pinching capillary discharge nitrogen plasma source. The emitted SXR radiation is filtered by a titanium foil and focused by an ellipsoidal condenser mirror into the sample plane. A Fresnel zone plate was used to create a transmission image of the sample onto a CCD camera. To assess the resolution of the microscope, a standard sample-copper mesh-was imaged. The spatial resolution of the microscope is 75 nm at half-pitch, calculated via a 10--90% intensity knife-edge test. The applicability of the microscope is demonstrated by the imaging of green algae--Desmodesmus c.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10306 - Optics (including laser optics and quantum optics)

Project

<a href="/en/project/LTT17015" target="_blank" >LTT17015: Reserch in frame International Center for Dense Magnetized Plasmas</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů