Melatonin receptor-dependent antiarrhythmic action in rat in vivo model of heart post-ischemic reperfusion
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F23%3A00371881" target="_blank" >RIV/68407700:21460/23:00371881 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/apha.14044" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/apha.14044</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Melatonin receptor-dependent antiarrhythmic action in rat in vivo model of heart post-ischemic reperfusion
Original language description
Introduction: Melatonin has been proven to have cardioprotective effects most likely through promoted expression of antioxidant enzymes and suppression of inflammation-related genes. Hence, this study aimed to explore receptor-dependent pathways responsible for the melatonin antiarrhythmic action in a rat model of post-ischemic heart reperfusion (Wistar male rats n = 86, 3-month-old). Methods: Ischemia was induced by the left coronary artery ligation for 5 min. Melatonin (4 mg/kg, i.v.), luzindole (blocker of melatonin receptors, 0.4 mg/kg, i.v.), and a combination of melatonin + luzindole were infused before reperfusion and in the baseline. Incidence of ventricular fibrillation (VF) and conduction velocity were measured in vivo. Left ventricular tissue was used for expression of electrical coupling protein, connexin-43 (Cx43) and markers of oxidative stress evaluation. Results: Melatonin prevented ischemia-related conduction slowing and decreased VF incidence that was associated with increased myocardial phosphorylated (P) Cx43 abundance. Luzindole abolished melatonin antiarrhythmic effect, down-regulated Cx43 and protein kinase Cε that phosphorylates Cx43. However, melatonin did not change oxidative stress markers. Conclusion: We can assume, that the antiarrhythmic effect of melatonin was mediated by the improved conduction properties associated with enhanced Cx43 phosphorylation, most likely independent from its antioxidant effect.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
30109 - Pathology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů