Proteomic Analysis of Plasma Proteins after Low-Level Laser Therapy in Rats
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21720%2F19%3A00346786" target="_blank" >RIV/68407700:21720/19:00346786 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16170/19:43878408
Result on the web
<a href="https://doi.org/10.33549/PHYSIOLRES.934377" target="_blank" >https://doi.org/10.33549/PHYSIOLRES.934377</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/PHYSIOLRES.934377" target="_blank" >10.33549/PHYSIOLRES.934377</a>
Alternative languages
Result language
angličtina
Original language name
Proteomic Analysis of Plasma Proteins after Low-Level Laser Therapy in Rats
Original language description
The laser radiation absorbed by cells induces production of reactive oxygen species (ROS), followed by the development of oxidative stress. Proteins are major targets for ROS due to their abundance in biological systems. The aim of the present pilot study was to examine the effects of transcutaneous laser blood irradiation (TLBI), i.e., low-level laser therapy (LLLT) at 830 nm on plasma proteome in Wistar rats. Rats were irradiated in the heart area (i.e. coronary arteries) daily (i.e., for 9-day period), by commercially available GaAsAl diode laser (Maestro/CCM, Medicom Prague, Czech Republic, λ=830 nm, power density 450mW/cm2, daily dose 60,3 J/ cm2, irradiation time 134 sec). The comparison of blood plasma proteome from irradiated and non-irradiated rats was performed utilizing 2D electrophoresis followed by MALDI TOF/TOF mass spectrometry. LLLT led to a quantitative change in the acute phase proteins with antioxidant protection i.e., haptoglobin (log2 fold change (FC)=3.5), hemopexin (log2 FC=0.5), fibrinogen gamma (log2 FC=1.4), alpha-1-antitrypsin (log2 FC=-2.2), fetuin A (log2 FC=-0.6) and fetuin B (log2 FC=-2.3). In comparison to conventional biochemical methods, the changes in protein levels in blood plasma induced by LLLT offer a deeper insight into the oxidative stress response.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)
Result continuities
Project
<a href="/en/project/LO1605" target="_blank" >LO1605: University Centre for Energy Efficient Buildings – Sustainability Phase</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiological Research
ISSN
0862-8408
e-ISSN
1802-9973
Volume of the periodical
68
Issue of the periodical within the volume
Suppl. 4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
6
Pages from-to
"S399"-"S404"
UT code for WoS article
000529413800005
EID of the result in the Scopus database
2-s2.0-85081138479