MiR-449a antagonizes EMT through IL-6-mediated trans-signaling in laryngeal squamous cancer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21720%2F24%3A00380335" target="_blank" >RIV/68407700:21720/24:00380335 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.omtn.2024.102140" target="_blank" >https://doi.org/10.1016/j.omtn.2024.102140</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.omtn.2024.102140" target="_blank" >10.1016/j.omtn.2024.102140</a>

Result language

angličtina

Original language name

MiR-449a antagonizes EMT through IL-6-mediated trans-signaling in laryngeal squamous cancer

Original language description

MicroRNAs (miRNAs) are involved in post-transcriptional gene expression regulation and in mechanisms of cancer growth and metastases. In this light, miRNAs could be promising therapeutic targets and biomarkers in clinical practice. Therefore, we investigated if specific miRNAs and their target genes contribute to laryngeal squamous cell carcinoma (LSCC) development. We found a significant decrease of miR-449a in LSCC patients with nodal metastases (63.3%) compared with patients without nodal involvement (44%). The AmpliSeq Transcriptome of HNO-210 miR-449a-transfected cell lines allowed the identification of IL6-R as a potential target. Moreover, the downregulation of IL6-R and the phosphorylation reduction of the downstream signaling effectors, suggested the inhibition of the IL-6 trans-signaling pathway. These biochemical effects were paralleled by a significant inhibition of invasion and migration in vitro and in vivo, supporting an involvement of epithelial-mesenchymal transition. These findings indicate that miR-449a contributes to suppress the metastasization of LSCC by the IL-6 trans-signaling block and affects sensitivity to external stimuli that mimic pro-inflammatory conditions.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mol Ther Nucleic Acids

ISSN

2162-2531

e-ISSN

2162-2531

Volume of the periodical

35

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

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UT code for WoS article

001207538200001

EID of the result in the Scopus database

2-s2.0-85185787879