Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F09%3A%230000187" target="_blank" >RIV/70565813:_____/09:#0000187 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR

Original language description

This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) The quantitative real-time Reverse Transcriptase PCR (qRT-PCR) assay based on a TaqMan probe wasdeveloped for a rapid detection and quantification of avian influenza virus RNA in clinical samples collected from mute swans. A recombinant plasmid containing the matrix protein gene amplicon was constructed for a quantitative assay of copy numbers of the target gene. Avian influenza virus A/Cygnus olor/Brno-cz/2006 was adapted to grow in VERO cells. In the same passage of cell cultivation, the concentration of viral RNA was determined to be 1.01 x 10(7) copies/ml and TCID50 was 10(4.2)/ml. From thesevalues the ratio of one RNA copy to 0.00157 virion capable of VERO cells infection was calculated. This ratio was used to estimate the virus concentrations in the tissues of dead mute swans

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

VETERINARNI MEDICINA

ISSN

0375-8427

e-ISSN

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Volume of the periodical

54

Issue of the periodical within the volume

9

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

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UT code for WoS article

000272392600007

EID of the result in the Scopus database

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