Use of the HRM Analysis in Pathogen Identification

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F18%3AN0000034" target="_blank" >RIV/70565813:_____/18:N0000034 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Use of the HRM Analysis in Pathogen Identification
Original language description
The HRM – high resolution melting – analysis represents a post-PCR method enabling the differentiation according to the sequence of the amplicon. Thus, the amplicon is analyzed right after the real-time PCR in the same machine without further manipulation with samples. The so-called third generation intercalating dyes are used in the reaction, and special software for the analysis of raw data is essential. To demonstrate the usefulness of the HRM analysis, tick-borne encephalitis virus (TBEV) and Brucella sp. were chosen as examples of pathogenic biological agents. The forth involves three distinct subtypes connected with a varied course of the disease, the latter comprises a high number of genetically closely related species, although some of them possess a high risk for humans. Accordingly, the HRM analysis was selected to distinguish B. abortus, B. melitensis and B. suis from the rest of Brucella sp.
Czech name
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Czech description
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Project
<a href="/en/project/VI20172019063" target="_blank" >VI20172019063: Development of New Methodologies for Detection of Biological Agents in Area of Observance of the Biological Weapons Convention</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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