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Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F04%3A63502562" target="_blank" >RIV/70883521:28110/04:63502562 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting

  • Original language description

    In our study we aimed to find the universal procedure which would enable us to get DGGE profiles from larger set of samples where the soils differed substantially in contents of organic matter. Fraction of humic substances displays similar properties toDNA hence it can be uneasy to remove them during DNA isolation process and the substances consequently inhibit PCR. Additional cleaning of isolated DNA appears to be necessary. for most of our samples, two cleanings of isolated DNA (Wizard DNA clean-up systém, Promega) and subsequent tenfold dilution were sufficient. The most problematic sample needed three cleaning steps and tenfold dilution. Our experiences imply that dilution is more useful than additional cleaning for some samples.

  • Czech name

    Isolace bakteriální DNA z velmi humosních stepních půd a následná DGGE Fingerprinting

  • Czech description

    Byl ověřován postup isolace bakteriální DNA z velmi humosních stepních půd. Huminové látky ztěžovaly isolaci DNA z půd i inhibovaly následnou proceduru PCR. Práce prokázala, že dodatečné přečištění získané DNA je nezbytné. Z některých typů vzorků je všaknemožné získat požadovanou DNA universálním postupem.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    9th Methodological workshop: Present Methods for Investigation of Microbial Community Biodiversity in Soils and Substrates

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    4

  • Pages from-to

    120-123

  • Publisher name

    Institut of Soil biology AS ČR

  • Place of publication

    České Budějovice

  • Event location

    České Budějovice

  • Event date

    Mar 2, 2004

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article