Micro-thermal field-flow fractionation of bacteria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F07%3A63505368" target="_blank" >RIV/70883521:28110/07:63505368 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Micro-thermal field-flow fractionation of bacteria
Original language description
The retention of Staphylococcus epidermidis bacteria cells, achieved with the use of micro-thermal field-flow fractionation and described in this paper, represents the first experimental proof that the separation and characterization of the bio-macromolecules and biological particles is possible by exploiting Ludwig-Soret effect of thermal diffusion. The experiments were carried out under gentle experimental conditions preventing the denaturation of the bacteria. Lift forces, appearing at high linear velocities of the carrier liquid, generated the focusing mechanism of the retention which resulted in high-speed and high-performance separation performed in less than 10 minutes.
Czech name
Mikrotermální field-flow frakcionace bakterií
Czech description
Metodou mikro-termální field-flow frakcionace bylo dosaženo separace bakterií Staphylococcus epidermidis. Je to vůbec první pokus separace a charakterizace biomakromolekul a biologických částic termální difúzí (Ludwig-Soret). Experimenty byly provedeny za přesně zvolených experimentálních podmínek, aby se zabránilo denaturaci bakterií. Vztlakové síly, které se objevují při vysokých rychlostech mobilní fáze, způsobily fokusační mechanismus separace, která byla kratší než 10 minut.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences
ISSN
1570-0232
e-ISSN
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Volume of the periodical
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Issue of the periodical within the volume
852
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
512-518
UT code for WoS article
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EID of the result in the Scopus database
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