Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F10%3A63510070" target="_blank" >RIV/70883521:28110/10:63510070 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences
Original language description
TGGE is an efficient method which allows us to study both composition and dynamics of complex microbial communities without the necessity of cultivation. Sample´s total DNA isolation and consequent PCR amplification of desired gene´s fragment need to becarried out before the TGGE experiment. Such pretreatment provides us with the population of gene fragments of the identical length from virtually all the strains present in the sample, and differing in their exact sequences. The DNA sequence variation in obtained gene fragments is a base for subsequent TGGE separation and identification of particular bacterial strains. Currently an equivalent method called denaturing gradient gel electrophoresis (DGGE) is used more often usually performed on D-code instrument (Bio-rad). In our laboratory we try to adopt TGGE instrumented on TGGE-Maxi system (Biometra) where the procedure is different particularly in the gel preparation and some other parameters. The instrument nowadays is relatively ra
Czech name
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Czech description
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Project
<a href="/en/project/GAP108%2F10%2F0200" target="_blank" >GAP108/10/0200: Biodegradation study of polymeric materials by combination of advanced methods</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
25. výročný kongres československej spoločnosti mikrobiologickej s medzinárodnou účasťou
ISBN
970-80-970477-8-8
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
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Publisher name
Československá spoločnosť mikrobiologická
Place of publication
Bratislava
Event location
Stará Lesná, Vysoké Tatry
Event date
Jan 1, 2010
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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