Versatile digital polymerase chain reaction chip design, fabrication, and image processing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28140%2F18%3A63520516" target="_blank" >RIV/70883521:28140/18:63520516 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26220/18:PU130843 RIV/68407700:21230/19:00325904
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0925400518321919" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0925400518321919</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.snb.2018.12.072" target="_blank" >10.1016/j.snb.2018.12.072</a>
Alternative languages
Result language
angličtina
Original language name
Versatile digital polymerase chain reaction chip design, fabrication, and image processing
Original language description
We present a family of digital polymerase chain reaction (dPCR) chips made of silicon that are (9 × 9) mm2 in size, have a unique layout design, and have between 26,448 and 1,656,000 reaction wells. The chip layout was split into six fields sized 4200 × 2785 μm2 that contain reaction wells with a gap of a few μm separating the fields from each other. We also developed a universal fluorescence imaging method capable of processing images from those chips. It comprised automatic image acquisition, image stitching, alignment mark registration, rotation correction, determination of reaction wells’ positions, and result processing. The chip design, with fully automated image processing, provided a fast and reliable system for the absolute quantitative analysis of dPCR. Combined with ease-of-use, this family of dPCR chips offers a pathway to enable fast and affordable digital microfluidic diagnostic applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)
Result continuities
Project
<a href="/en/project/GA16-11140S" target="_blank" >GA16-11140S: Microfludics-based Ultra Fast Differential Scanning Fluorimetry for Drug Discovery (µDSF)</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
SENSORS AND ACTUATORS B-CHEMICAL
ISSN
0925-4005
e-ISSN
—
Volume of the periodical
2018
Issue of the periodical within the volume
283
Country of publishing house
CH - SWITZERLAND
Number of pages
8
Pages from-to
677-684
UT code for WoS article
000455854000082
EID of the result in the Scopus database
2-s2.0-85058703025