The Effectiveness of ddPCR for Detection of Point Mutations in Poor-Quality Saliva Samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F71009396%3A_____%2F22%3AN0000020" target="_blank" >RIV/71009396:_____/22:N0000020 - isvavai.cz</a>
Alternative codes found
RIV/61988987:17110/22:A2302FHA
Result on the web
<a href="https://www.mdpi.com/2227-9032/10/5/947" target="_blank" >https://www.mdpi.com/2227-9032/10/5/947</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/healthcare10050947" target="_blank" >10.3390/healthcare10050947</a>
Alternative languages
Result language
angličtina
Original language name
The Effectiveness of ddPCR for Detection of Point Mutations in Poor-Quality Saliva Samples
Original language description
Background: The noninvasive collection of saliva samples for DNA analyses is simple, and its potential for research and diagnostic purposes is great. However, DNA isolates from such samples are often of inferior quality to those from blood. Aim: The aim of this study was to investigate the robustness and sensitivity of the ddPCR instrument for genetic analyses from saliva samples of poor quality by comparing their results to those obtained using an established method from blood samples. Methods: Blood and saliva were collected from 47 university students, which was followed by manual isolation of DNA and analysis on droplet digital PCR (ddPCR). Results of analyses were supplemented with values of fractional abundances. Results: ddPCR proved to be highly suitable for analysis of even low-quality saliva samples (concentrations as low as 0.79 ng/mu L), especially when augmented by fractional abundance data. This combination yielded 100% agreement with results obtained from blood samples. Conclusion: This study verified the applicability of ddPCR as a sensitive and robust method of genetic diagnostic testing even from low-quality saliva isolates. This makes it potentially suitable for a wide range of applications and facilitates the performance of large epidemiological studies, even if sampling or sample processing is suboptimal.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30304 - Public and environmental health
Result continuities
Project
<a href="/en/project/EF16_019%2F0000798" target="_blank" >EF16_019/0000798: Healthy Aging in Industrial Environment</a><br>
Continuities
O - Projekt operacniho programu
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Healthcare
ISSN
2227-9032
e-ISSN
2227-9032
Volume of the periodical
10
Issue of the periodical within the volume
947
Country of publishing house
CH - SWITZERLAND
Number of pages
8
Pages from-to
1-8
UT code for WoS article
000801558700001
EID of the result in the Scopus database
2-s2.0-85131051764