Can the assessment of ABCB1 gene expression predict its function in vitro?
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F15%3A00010929" target="_blank" >RIV/75010330:_____/15:00010929 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/15:73585348
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/ejh.12470/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/ejh.12470/abstract</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ejh.12470" target="_blank" >10.1111/ejh.12470</a>
Alternative languages
Result language
angličtina
Original language name
Can the assessment of ABCB1 gene expression predict its function in vitro?
Original language description
Increased expression of the ABCB1 gene in cancer cells is usually connected with occurrence of multidrug resistance (MDR) and poor prognosis. However, the correlation between ABCB1 expression and MDR phenotype is difficult to prove in clinical samples. Most of the researchers believe that these difficulties are due to the poor reliability and sensitivity of assays for detection of ABCB1 expression in clinical samples. However, the complexity of P-gp mediated resistance cannot be reduced to the methodical difficulties only. Here, we addressed the question how widely used methods for detection of ABCB1 expression levels could predict its functional activity and thus its contribution to drug resistance in defined conditions in vitro. The ABCB1 expressionwas assessed at the mRNA level by quantitative real-time polymerase chain reaction (qRT-PCR), and at the protein level by flow cytometry using UIC2 antibody. The ABCB1 function was monitored using a calcein AM accumulation assay. We obser
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP303%2F12%2FG163" target="_blank" >GBP303/12/G163: Centre of drug-dietary supplements interactions and nutrigenetics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Haematology
ISSN
0902-4441
e-ISSN
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Volume of the periodical
95
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
150-159
UT code for WoS article
000357694500007
EID of the result in the Scopus database
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