Virulence factor cargo and host cell interactions of Shiga toxin-producing Escherichia coli outer membrane vesicles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F21%3A00013659" target="_blank" >RIV/75010330:_____/21:00013659 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007%2F978-1-0716-1339-9_8" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-0716-1339-9_8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-1339-9_8" target="_blank" >10.1007/978-1-0716-1339-9_8</a>
Alternative languages
Result language
angličtina
Original language name
Virulence factor cargo and host cell interactions of Shiga toxin-producing Escherichia coli outer membrane vesicles
Original language description
Outer membrane vesicles (OMVs), nanoparticles released by Shiga toxin-producing Escherichia coli (STEC), have been identified as novel efficient virulence tools of these pathogens. STEC O157 OMVs carry a cocktail of virulence factors including Shiga toxin 2a (Stx2a), cytolethal distending toxin V (CdtV), EHEC hemolysin, flagellin, and lipopolysaccharide. OMVs are taken up by human intestinal epithelial and microvascular endothelial cells, the major targets during STEC infection, and deliver the virulence factors into host cells. There the toxins separate from OMVs and are trafficked via different pathways to their target compartments, i.e., the cytosol (Stx2a-A subunit), nucleus (CdtV-B subunit), and mitochondria (EHEC hemolysin). This leads to a toxin-specific host cell injury and ultimately apoptotic cell death. Besides their cytotoxic effects, STEC OMVs trigger an inflammatory response via their lipopolysaccharide and flagellin components. In this chapter, we describe methods for the isolation and purification of STEC OMVs, for the detection of OMV-associated virulence factors, and for the analysis of OMV interactions with host cells including OMV cellular uptake and intracellular trafficking of OMVs and OMV-delivered toxins.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Shiga Toxin-Producing E. coli
ISBN
978-1-0716-1338-2
Number of pages of the result
29
Pages from-to
177-205
Number of pages of the book
436
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
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