Whole genome sequencing of Neisseria meningitidis Y isolates collected in the Czech Republic in 1993-2018

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F22%3A00014077" target="_blank" >RIV/75010330:_____/22:00014077 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11120/22:43923057

Result on the web

<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0265066" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0265066</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0265066" target="_blank" >10.1371/journal.pone.0265066</a>

Result language

angličtina

Original language name

Whole genome sequencing of Neisseria meningitidis Y isolates collected in the Czech Republic in 1993-2018

Original language description

Introduction: The study presents the analysis of whole genome sequencing (WGS) data for Neisseria meningitidis serogroup Y isolates collected in the Czech Republic and their comparison to other countries. The aim of the study was to determine whether there are lineages of N. meningitidis serogroup Y in the Czech Republic genetically related to foreign ones that have been causing an increase of the morbidity and the mortality of invasive meningococcal disease (IMD) world-wide recently. Material and methods: The WGS data of 43 Czech N. meningitidis Y isolates, 35 from IMD and 8 from healthy carriers were analysed. Due to the potential of meningococcal B vaccines to induce protection against non-B serogroups, the coverage of Czech isolates of N. meningitidis Y by these vaccines was studied. The WGS data of Czech, European and non-European isolates of N. meningitidis serogroup Y were compared. Results: WGS assigned 36 isolates of N. meningitidis Y to five clonal complexes: cc23, cc92, cc167, cc103, and cc174, while seven isolates remained unassigned to any clonal complexes (ccUA). Eighteen invasive isolates belonged to clonal complex cc23, which was detected throughout the studied years. The occurrence of cc23 was recorded in all age groups of IMD patients, with the highest found in those aged 15-19 years. On the phylogenetic network isolates of cc23 form a separate lineage, distinct from all other isolates of N. meningitidis Y. The remaining isolates were assigned to other clonal complexes and have very low relatedness to cc23 isolates and to each other. The comparison with foreign WGS data showed that within the main genetic lineages, which are defined by clonal complexes, Czech isolates of N. meningitidis Y, similar to European ones, mostly cluster together and form geographical sublineages. Conclusions: WGS analysis showed the population of Czech N. meningitidis Y isolates as relatively heterogeneous, containing a large number of genetic lineages. The Czech isolates of N. meningitidis Y follow the trend observed for European isolates. Our result was one of the bases for updating the recommended vaccination strategy in the Czech Republic.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30303 - Infectious Diseases

Project

<a href="/en/project/NV19-09-00319" target="_blank" >NV19-09-00319: Study of meningococcal population by the whole genome sequencing method - base for update of vaccination strategy</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS One

ISSN

1932-6203

e-ISSN

—

Volume of the periodical

17

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

18

Pages from-to

e0265066

UT code for WoS article

000820912700084

EID of the result in the Scopus database

2-s2.0-85126317858