Proposal of Acinetobacter thermotolerans sp. nov. to accommodate bovine feces-dwelling bacteria growing at 47 ° C

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F24%3A00014739" target="_blank" >RIV/75010330:_____/24:00014739 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/24:10487765 RIV/00064203:_____/24:10487765

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0723202024000742?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0723202024000742?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.syapm.2024.126560" target="_blank" >10.1016/j.syapm.2024.126560</a>

Result language

angličtina

Original language name

Proposal of Acinetobacter thermotolerans sp. nov. to accommodate bovine feces-dwelling bacteria growing at 47 ° C

Original language description

This study aimed to determine the taxonomic status of a new group of bovine strains of the genus Acinetobacter characterized by the rare ability to grow at temperatures above 44 degrees C. Initially, 24 strains were isolated from cattle feces collected at 11 farms in Czechia in 2022, representing a tentative new species based on preliminary whole-cell MALDI-TOF MS identification and rpoB gene sequencing. Twelve strains encompassing the within-group diversity were studied in detail, including whole genome de novo sequencing. Core genome-based phylogenetic analysis revealed that the 12 strains form a distinct clade within the genus. The genomic average nucleotide identity based on BLAST (ANIb) values within the clade were >= 98.2%, whereas the ANIb values between the clade and the known Acinetobacter species were < 83%. Each of the 12 genomes consisted of a circular chromosome (3.05-3.28 Mb) and 1-6 extrachromosomal elements (1.43-122.5 kb). All strains were non-glucose oxidizing, non-hemolytic, non-proteolytic, and prototrophic, and used acetate, ethanol, and DL-lactate as sole carbon and energy sources. Unrestricted growth at 47 degrees C was their unique diagnostic characteristic in a genus-wide comparative analysis and their growth kinetics at 37-46 degrees C differed from that of Acinetobacter baumannii. The studied strains were susceptible to 18 antibiotics except for sporadic resistance to streptomycin, tetracycline, and sulfonamides associated with the presence of strA/strB, tet(Y), and sul2 genes, respectively. We conclude that the studied strains represent a new species, for which we propose the name Acinetobacter thermotolerans sp. nov. The type strain is ANC 7454(T) (= CCM 9356(T) = CCUG 77195(T) = CNCTC 8200(T)).

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA22-05373S" target="_blank" >GA22-05373S: Cattle excrements and manure as a reservoir of acinetobacters representing risk to human health</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Systematic and Applied Microbiology

ISSN

0723-2020

e-ISSN

1618-0984

Volume of the periodical

47

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

126560

UT code for WoS article

001361638500001

EID of the result in the Scopus database

2-s2.0-85209071814