Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F09%3A00330614" target="_blank" >RIV/86652036:_____/09:00330614 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins
Original language description
Recognition and binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. This is the topic of many scientific groups, and so far many molecules on the sperm surface, involved in mentioned process, were identified.Sperm surface proteins of ejaculated and capacitated boar sperms were isolated by Pierce Cell Surface Isolation Kit and characterized by 1D, 2D electrophoreses, glycoprotein staining, hyaluronidase and proteolytic activities. Finally binding assay withZP was performed. Results have shown the quantitative and qualitative differences in both 1D and 2D electrophoretic profiles, glycoprotein profile was similar to 1D electrophoretic one. Neither hyaluronidase nor proteolytic activities were observed. Binding assay proved that interactions occur between both ejaculated and capacitated sperms. Protein interactions of capacitated sperm with ZP glycoproteins seem to be more specific.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů