Assessing oestrogenic effects of brominated flame retardants Hexabromocyclododecane and Tetrabromobisphenol A on MCF-7 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F10%3A00356209" target="_blank" >RIV/86652036:_____/10:00356209 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Assessing oestrogenic effects of brominated flame retardants Hexabromocyclododecane and Tetrabromobisphenol A on MCF-7 cells

Original language description

We used the proliferation test (E-screen assay) in MCF-7 breast cancer cells and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis of TFF1 gene expression to analyze estrogenicity of the studied compounds. RT-qPCR has provento be a fast and valuable molecular technique in gene expression quantification. HBCD but not TBBPA increased cell proliferation in MCF-7 cells and up-regulates TFF1 gene expression in a concentration-dependent manner. Anti-estrogen ICI 182,780 inhibitsup-regulation of TFF1 by HBCD. We showed that HBCD displays estrogen-like effects on MCF-7 cells. TBBPA, on the other hand, has not shown any estrogenic effect mediated by the estrogen receptor alpha.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

DN - Environmental impact on health

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Biologica

ISSN

0015-5500

e-ISSN

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Volume of the periodical

56

Issue of the periodical within the volume

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Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

35-39

UT code for WoS article

000288623700007

EID of the result in the Scopus database

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