Ubiquitin-activating enzyme (UBA1) is required for sperm capacitation, acrosomal exocytosis and sperm-egg coat penetration during porcine fertilization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F12%3A00367613" target="_blank" >RIV/86652036:_____/12:00367613 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/j.1365-2605.2011.01217.x" target="_blank" >http://dx.doi.org/10.1111/j.1365-2605.2011.01217.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1365-2605.2011.01217.x" target="_blank" >10.1111/j.1365-2605.2011.01217.x</a>
Alternative languages
Result language
angličtina
Original language name
Ubiquitin-activating enzyme (UBA1) is required for sperm capacitation, acrosomal exocytosis and sperm-egg coat penetration during porcine fertilization
Original language description
Protein ubiquitination is covalent post-translational modification that targets proteins for proteolysis by the 26S proteasome. The E1-type ubiquitin-activating enzyme (UBA1) is responsible for the initial step of ubiquitin?protein ligation. Proteasomalproteolysis of ubiquitinated spermatozoa and oocyte proteins occurs during mammalian fertilization. UBA1 was detected in boar sperm-acrosomal extracts by Western blotting (WB) and by IMF in the acrosomal caps. A specific UBA1 inhibitor, PYR-41, altered the outer acrosomal membrane during sperm capacitation and reduced fertilization rates during in vitro fertilization. WB with antiphosphotyrosine antibodies and antibodies against acrosomal proteins SPINK2 (acrosin inhibitor) and AQN1 (spermadhesin) revealed that the capacitationinduced modification of those proteins was altered by PYR-41. It appears that de novo protein ubiquitination involving UBA1 contributes to sperm capacitation and acrosomal function during fertilization.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Andrology
ISSN
0105-6263
e-ISSN
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Volume of the periodical
35
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
196-210
UT code for WoS article
000301442200012
EID of the result in the Scopus database
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