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Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F12%3A00376560" target="_blank" >RIV/86652036:_____/12:00376560 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11140/12:10296031

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.cca.2012.01.015" target="_blank" >http://dx.doi.org/10.1016/j.cca.2012.01.015</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.cca.2012.01.015" target="_blank" >10.1016/j.cca.2012.01.015</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis

  • Original language description

    Background: Although important improvements of downstream molecular in vitro diagnostics assays based on RNA from blood were made, the pre-analytical workflow is still poorly defined. Methods: We performed a multicenter study within the EU-granted SPIDIAproject to investigate blood collection and shipping influence on the following RNA quality parameters: yield, purity, integrity, RT-qPCR interference and IL1B, IL8, FOS and GAPDH gene expression. Two models were designed: Exp A. Ten laboratories collected blood from an own donor into two different tubes (with or without stabilizer) and extracted RNA at two different times; Exp B. Blood was drawn from a single donor and shipped to ten laboratories in two different tubes (with or without stabilizer) forRNA extraction. Results: In both models and collection tubes, reliable results were obtained for purity, yield, GAPDH expression, and interferences. A substantial variation in RIN (Exp A) and in transcription levels of IL1B. IL8 and FOS

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Clinica Chimica Acta

  • ISSN

    0009-8981

  • e-ISSN

  • Volume of the periodical

    413

  • Issue of the periodical within the volume

    7-8

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    8

  • Pages from-to

    779-786

  • UT code for WoS article

    000301908000023

  • EID of the result in the Scopus database