Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F12%3A00376560" target="_blank" >RIV/86652036:_____/12:00376560 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11140/12:10296031
Result on the web
<a href="http://dx.doi.org/10.1016/j.cca.2012.01.015" target="_blank" >http://dx.doi.org/10.1016/j.cca.2012.01.015</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cca.2012.01.015" target="_blank" >10.1016/j.cca.2012.01.015</a>
Alternative languages
Result language
angličtina
Original language name
Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis
Original language description
Background: Although important improvements of downstream molecular in vitro diagnostics assays based on RNA from blood were made, the pre-analytical workflow is still poorly defined. Methods: We performed a multicenter study within the EU-granted SPIDIAproject to investigate blood collection and shipping influence on the following RNA quality parameters: yield, purity, integrity, RT-qPCR interference and IL1B, IL8, FOS and GAPDH gene expression. Two models were designed: Exp A. Ten laboratories collected blood from an own donor into two different tubes (with or without stabilizer) and extracted RNA at two different times; Exp B. Blood was drawn from a single donor and shipped to ten laboratories in two different tubes (with or without stabilizer) forRNA extraction. Results: In both models and collection tubes, reliable results were obtained for purity, yield, GAPDH expression, and interferences. A substantial variation in RIN (Exp A) and in transcription levels of IL1B. IL8 and FOS
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinica Chimica Acta
ISSN
0009-8981
e-ISSN
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Volume of the periodical
413
Issue of the periodical within the volume
7-8
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
779-786
UT code for WoS article
000301908000023
EID of the result in the Scopus database
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