2 days Hands-on qPCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F13%3A00425509" target="_blank" >RIV/86652036:_____/13:00425509 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

2 days Hands-on qPCR

Original language description

Day 1 - This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains: Basic PCR theory. The theory of real-time PCR. Applications and possibilities of qPCR. Comparison of qPCR with regular PCR. Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc). Different instrument platforms and their typical uses. Primer design. Theproblem of primer-dimer formation and how to minimize them. Probe design. Experimental design and optimization. Basic data handling and analysis. Day 2 - Introduction to quantification principles. Quantification strategies, uses and limitations. Calculations using different relative quantification methods. Strategies for normalization of qPCR data. In situ calibration for compensation of inhibition in samples. Absolute quantification.

Czech name

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Czech description

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Type

W - Workshop organization

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Event location

Praha, BTÚ

Event country

CZ - CZECH REPUBLIC

Event starting date

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Event ending date

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Total number of attendees

7

Foreign attendee count

3

Type of event by attendee nationality

WRD - Celosvětová akce