Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F14%3A00427908" target="_blank" >RIV/86652036:_____/14:00427908 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/14:00432511
Result on the web
<a href="http://dx.doi.org/10.1155/2014/138350" target="_blank" >http://dx.doi.org/10.1155/2014/138350</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2014/138350" target="_blank" >10.1155/2014/138350</a>
Alternative languages
Result language
angličtina
Original language name
Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture
Original language description
This study aimed to provide a molecular signature for enriched adult human stem/progenitor spermatogonia during short-term (<2 weeks) and long-term culture (up to more than 14 months) in comparison to human testicular fibroblasts and human embryonic stemcells. Human spermatogonia were isolated by CD49f magnetic activated cell sorting and collagen(-)/laminin(+) matrix binding from primary testis cultures obtained from ten adult men. For transcriptomic analysis, single spermatogonia-like cells were collected based on their morphology and dimensions using a micromanipulation system from the enriched germ cell cultures. Immunocytochemical, RT-PCR and microarray analyses revealed that the analyzed populations of cells were distinct at the molecular level.The germ-and pluripotency-associated genes and genes of differentiation/spermatogenesis pathway were highly expressed in enriched short-term cultured spermatogonia. After long-term culture, a proportion of cells retained and aggravated th
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BioMed Research International
ISSN
2314-6133
e-ISSN
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Volume of the periodical
2014
Issue of the periodical within the volume
138350
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
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UT code for WoS article
000333307400001
EID of the result in the Scopus database
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