Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F14%3A00427908" target="_blank" >RIV/86652036:_____/14:00427908 - isvavai.cz</a>

Alternative codes found

RIV/86652036:_____/14:00432511

Result on the web

<a href="http://dx.doi.org/10.1155/2014/138350" target="_blank" >http://dx.doi.org/10.1155/2014/138350</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2014/138350" target="_blank" >10.1155/2014/138350</a>

Result language

angličtina

Original language name

Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture

Original language description

This study aimed to provide a molecular signature for enriched adult human stem/progenitor spermatogonia during short-term (<2 weeks) and long-term culture (up to more than 14 months) in comparison to human testicular fibroblasts and human embryonic stemcells. Human spermatogonia were isolated by CD49f magnetic activated cell sorting and collagen(-)/laminin(+) matrix binding from primary testis cultures obtained from ten adult men. For transcriptomic analysis, single spermatogonia-like cells were collected based on their morphology and dimensions using a micromanipulation system from the enriched germ cell cultures. Immunocytochemical, RT-PCR and microarray analyses revealed that the analyzed populations of cells were distinct at the molecular level.The germ-and pluripotency-associated genes and genes of differentiation/spermatogenesis pathway were highly expressed in enriched short-term cultured spermatogonia. After long-term culture, a proportion of cells retained and aggravated th

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BioMed Research International

ISSN

2314-6133

e-ISSN

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Volume of the periodical

2014

Issue of the periodical within the volume

138350

Country of publishing house

US - UNITED STATES

Number of pages

17

Pages from-to

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UT code for WoS article

000333307400001

EID of the result in the Scopus database

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