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Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F14%3A00427908" target="_blank" >RIV/86652036:_____/14:00427908 - isvavai.cz</a>

  • Alternative codes found

    RIV/86652036:_____/14:00432511

  • Result on the web

    <a href="http://dx.doi.org/10.1155/2014/138350" target="_blank" >http://dx.doi.org/10.1155/2014/138350</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1155/2014/138350" target="_blank" >10.1155/2014/138350</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture

  • Original language description

    This study aimed to provide a molecular signature for enriched adult human stem/progenitor spermatogonia during short-term (<2 weeks) and long-term culture (up to more than 14 months) in comparison to human testicular fibroblasts and human embryonic stemcells. Human spermatogonia were isolated by CD49f magnetic activated cell sorting and collagen(-)/laminin(+) matrix binding from primary testis cultures obtained from ten adult men. For transcriptomic analysis, single spermatogonia-like cells were collected based on their morphology and dimensions using a micromanipulation system from the enriched germ cell cultures. Immunocytochemical, RT-PCR and microarray analyses revealed that the analyzed populations of cells were distinct at the molecular level.The germ-and pluripotency-associated genes and genes of differentiation/spermatogenesis pathway were highly expressed in enriched short-term cultured spermatogonia. After long-term culture, a proportion of cells retained and aggravated th

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    BioMed Research International

  • ISSN

    2314-6133

  • e-ISSN

  • Volume of the periodical

    2014

  • Issue of the periodical within the volume

    138350

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    17

  • Pages from-to

  • UT code for WoS article

    000333307400001

  • EID of the result in the Scopus database