Characterization of tetraspanin protein CD81 in mouse spermatozoa and bovine gametes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F16%3A00472835" target="_blank" >RIV/86652036:_____/16:00472835 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/16:00472835 RIV/00216208:11310/16:10329580

Result on the web

<a href="http://dx.doi.org/10.1530/REP-16-0304" target="_blank" >http://dx.doi.org/10.1530/REP-16-0304</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1530/REP-16-0304" target="_blank" >10.1530/REP-16-0304</a>

Result language

angličtina

Original language name

Characterization of tetraspanin protein CD81 in mouse spermatozoa and bovine gametes

Original language description

Sperm-egg interaction and fusion represent a key moment of fertilization. In mammals, it is not possible without the interaction of the tetraspanin superfamily proteins including CD81. A detailed immunohistochemical localization of CD81 was monitored in bovine oocytes during different maturation stages, as well as during early embryogenesis. In addition, characterization of CD81 was carried out in bovine and mouse sperm. In bovine eggs, CD81 was detected on the plasma membrane of the germinal vesicle, metaphase I and metaphase II oocytes. During fertilization, accumulation of CD81 molecules in the perivitelline space of fertilized oocytes, which appeared as vesicles associated with plasma membrane, was observed. In majority of bull-ejaculated sperm and caput, corpus and cauda epididymal sperm, as well as mouse cauda epididymal sperm, CD81 was found on the plasma membrane covering the apical acrosome. Although the process of capacitation did not influence the localization of CD81, it was lost from the surface of the acrosome-reacted spermatozoa in bull, in contrast to mouse sperm where there was a relocalization of the CD81 protein during acrosome reaction across the equatorial segment and later over the whole sperm head. The presented results highlight conservative unifying aspects of CD81 expression between cattle and mouse, together with mouse-specific traits in sperm CD81 behaviour, which emphasizes certain species-specific mechanisms of fertilization to be considered.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Reproduction

ISSN

1470-1626

e-ISSN

—

Volume of the periodical

152

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

785-793

UT code for WoS article

000387771600025

EID of the result in the Scopus database

2-s2.0-84996635642