Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F17%3A00481503" target="_blank" >RIV/86652036:_____/17:00481503 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/17:10369009

Result on the web

<a href="http://dx.doi.org/10.1016/j.freeradbiomed.2017.07.033" target="_blank" >http://dx.doi.org/10.1016/j.freeradbiomed.2017.07.033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.freeradbiomed.2017.07.033" target="_blank" >10.1016/j.freeradbiomed.2017.07.033</a>

Result language

angličtina

Original language name

Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death

Original language description

Mitochondrial electron transport chain (ETC) targeting shows a great promise in cancer therapy. It is particularly effective in tumors with high ETC activity where ETC-derived reactive oxygen species (ROS) are efficiently induced. Why modern ETC-targeted compounds are tolerated on the organismal level remains unclear. As most somatic cells are in non-proliferative state, the features associated with the ETC in quiescence could account for some of the specificity observed. Here we report that quiescent cells, despite increased utilization of the ETC and enhanced supercomplex assembly, are less susceptible to cell death induced by ETC disruption when glucose is not limiting. Mechanistically, this is mediated by the increased detoxification of ETC-derived ROS by mitochondrial antioxidant defense, principally by the superoxide dismutase 2 thioredoxin axis. In contrast, under conditions of glucose limitation, cell death is induced preferentially in quiescent cells and is correlated with intracellular ATP depletion but not with ROS. This is related to the inability of quiescent cells to compensate for the lost mitochondrial ATP production by the upregulation of glucose uptake. Hence, elevated ROS, not the loss of mitochondrially-generated ATP, are responsible for cell death induction by ETC disruption in ample nutrients condition, e.g. in well perfused healthy tissues, where antioxidant defense imparts specificity. However, in conditions of limited glucose, e.g. in poorly perfused tumors, ETC disruption causes rapid depletion of cellular ATP, optimizing impact towards tumor-associated dormant cells. In summary, we propose that antioxidant defense in quiescent cells is aided by local glucose limitations to ensure selectivity of ETC inhibition-induced cell death.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Free Radical Biology and Medicine

ISSN

0891-5849

e-ISSN

—

Volume of the periodical

112

Issue of the periodical within the volume

NOV 2017

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

253-266

UT code for WoS article

000411829300022

EID of the result in the Scopus database

2-s2.0-85026789016