3 days Hands-on qPCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F17%3A00483576" target="_blank" >RIV/86652036:_____/17:00483576 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

3 days Hands-on qPCR

Original language description

Day 1. Introduction to PCR and qPCR. qPCR data evaluation. Primer and probe design. Optimization of qPCR protocols. Hands-on lab running qPCR analyzing unknown samples with standard curve. Day 2. Normalization. Basic quantification theory. Absolute quantification strategies. Validation of assays. Quantification calculation examples. Hands-on lab running qPCR and calculating relative quantities with different strategies. Day 3. Sample preparation and isolation. Reverse transcription, RT. The MIQE guidelines. Group discussion

Czech name

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Czech description

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Type

W - Workshop organization

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

<a href="/en/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Event location

Vestec, BTÚ

Event country

CZ - CZECH REPUBLIC

Event starting date

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Event ending date

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Total number of attendees

10

Foreign attendee count

4

Type of event by attendee nationality

WRD - Celosvětová akce