Technical aspects and recommendations for single-cell qPCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F18%3A00503182" target="_blank" >RIV/86652036:_____/18:00503182 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.mam.2017.07.004" target="_blank" >http://dx.doi.org/10.1016/j.mam.2017.07.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mam.2017.07.004" target="_blank" >10.1016/j.mam.2017.07.004</a>

Result language

angličtina

Original language name

Technical aspects and recommendations for single-cell qPCR

Original language description

Single cells are basic physiological and biological units that can function individually as well as in groups in tissues and organs. It is central to identify, characterize and profile single cells at molecular level to be able to distinguish different kinds, to understand their functions and determine how they interact with each other. During the last decade several technologies for single-cell profiling have been developed and used in various applications, revealing many novel findings. Quantitative PCR (qPCR) is one of the most developed methods for single-cell profiling that can be used to interrogate several analytes, including DNA, RNA and protein. Single-cell qPCR has the potential to become routine methodology but the technique is still challenging, as it involves several experimental steps and few molecules are handled. Here, we discuss technical aspects and provide recommendation for single-cell qPCR analysis. The workflow includes experimental design, sample preparation, single-cell collection, direct lysis, reverse transcription, preamplification, qPCR and data analysis. Detailed reporting and sharing of experimental details and data will promote further development and make validation studies possible. Efforts aiming to standardize single-cell qPCR open up means to move single-cell analysis from specialized research settings to standard research laboratories. (C) 2017 Elsevier Ltd. All rights reserved.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Aspects of Medicine

ISSN

0098-2997

e-ISSN

—

Volume of the periodical

59

Issue of the periodical within the volume

FEB 2018

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

28-35

UT code for WoS article

000423056300003

EID of the result in the Scopus database

2-s2.0-85026450067