Kinetically distinct phases of tau on microtubules regulate kinesin motors and severing enzymes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F19%3A00521183" target="_blank" >RIV/86652036:_____/19:00521183 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41556-019-0374-6" target="_blank" >https://www.nature.com/articles/s41556-019-0374-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41556-019-0374-6" target="_blank" >10.1038/s41556-019-0374-6</a>

Result language

angličtina

Original language name

Kinetically distinct phases of tau on microtubules regulate kinesin motors and severing enzymes

Original language description

Tau is an intrinsically disordered protein, which diffuses on microtubules(1). In neurodegenerative diseases, collectively termed tauopathies, malfunction of tau and its detachment from axonal microtubules are correlated with axonal degeneration(2). Tau can protect microtubules from microtubuledegrading enzymes such as katanin(3). However, how tau carries out this regulatory function is still unclear. Here, using in vitro reconstitution, we show that tau molecules on microtubules cooperatively form cohesive islands that are kinetically distinct from tau molecules that individually diffuse on microtubules. Dependent on the tau concentration in solution, the islands reversibly grow or shrink by addition or release of tau molecules at their boundaries. Shielding microtubules from kinesin-1 motors and katanin, the islands exhibit regulatory qualities distinct from a comparably dense layer of diffusible tau. Superprocessive kinesin-8 motors penetrate the islands and cause their disassembly. Our results reveal a microtubule-dependent phase of tau that constitutes an adaptable protective layer on the microtubule surface. We anticipate that other intrinsically disordered axonal proteins display a similar cooperative behaviour and potentially compete with tau in regulating access to the microtubule surface.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Cell Biology

ISSN

1465-7392

e-ISSN

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Volume of the periodical

21

Issue of the periodical within the volume

9

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

1086-1092

UT code for WoS article

000484363700007

EID of the result in the Scopus database

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