Combined Atoh1 and Neurod1 Deletion Reveals Autonomous Growth of Auditory Nerve Fibers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F20%3A00541345" target="_blank" >RIV/86652036:_____/20:00541345 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs12035-020-02092-0" target="_blank" >https://link.springer.com/article/10.1007%2Fs12035-020-02092-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12035-020-02092-0" target="_blank" >10.1007/s12035-020-02092-0</a>
Alternative languages
Result language
angličtina
Original language name
Combined Atoh1 and Neurod1 Deletion Reveals Autonomous Growth of Auditory Nerve Fibers
Original language description
Ear development requires the transcription factors ATOH1 for hair cell differentiation and NEUROD1 for sensory neuron development. In addition, NEUROD1 negatively regulatesAtoh1gene expression. As we previously showed that deletion of theNeurod1gene in the cochlea results in axon guidance defects and excessive peripheral innervation of the sensory epithelium, we hypothesized that some of the innervation defects may be a result of abnormalities in NEUROD1 and ATOH1 interactions. To characterize the interdependency of ATOH1 and NEUROD1 in inner ear development, we generated a newAtoh1/Neurod1double null conditional deletion mutant. Through careful comparison of the effects of singleAtoh1orNeurod1gene deletion with combined doubleAtoh1andNeurod1deletion, we demonstrate that NEUROD1-ATOH1 interactions are not important for theNeurod1null innervation phenotype. We report that neurons lackingNeurod1can innervate the flat epithelium without any sensory hair cells or supporting cells left afterAtoh1deletion, indicating that neurons withNeurod1deletion do not require the presence of hair cells for axon growth. Moreover, transcriptome analysis identified genes encoding axon guidance and neurite growth molecules that are dysregulated in theNeurod1deletion mutant. Taken together, we demonstrate that much of the projections of NEUROD1-deprived inner ear sensory neurons are regulated cell-autonomously.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Neurobiology
ISSN
0893-7648
e-ISSN
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Volume of the periodical
57
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
5307-5323
UT code for WoS article
000565831800001
EID of the result in the Scopus database
2-s2.0-85090165942