3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F20%3A00541406" target="_blank" >RIV/86652036:_____/20:00541406 - isvavai.cz</a>
Result on the web
<a href="https://elifesciences.org/articles/62043" target="_blank" >https://elifesciences.org/articles/62043</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7554/eLife.62043" target="_blank" >10.7554/eLife.62043</a>

Result language
angličtina
Original language name
3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice
Original language description
Out of millions of ejaculated sperm, a few reach the fertilization site in mammals. Flagellar Ca2+ signaling nanodomains, organized by multi-subunit CatSper calcium channel complexes, are pivotal for sperm migration in the female tract, implicating CatSper-dependent mechanisms in sperm selection. Here using biochemical and pharmacological studies, we demonstrate that CatSper1 is an O-linked glycosylated protein, undergoing capacitation-induced processing dependent on Ca2+ and phosphorylation cascades. CatSper1 processing correlates with protein tyrosine phosphorylation (pY) development in sperm cells capacitated in vitro and in vivo. Using 3D in situ molecular imaging and ANN-based automatic detection of sperm distributed along the cleared female tract, we demonstrate that spermatozoa past the utero-tubal junction possess the intact CatSper1 signals. Together, we reveal that fertilizing mouse spermatozoa in situ are characterized by intact CatSper channel, lack of pY, and reacted acrosomes. These findings provide molecular insight into sperm selection for successful fertilization in the female reproductive tract.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Project
<a href="/en/project/GJ20-17403Y" target="_blank" >GJ20-17403Y: Effect of Estrogens on Testicular/Sperm Epigenome and Early Embryonic Development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)