Stable tug-of-war between kinesin-1 and cytoplasmic dynein upon different ATP and roadblock concentrations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F20%3A00541407" target="_blank" >RIV/86652036:_____/20:00541407 - isvavai.cz</a>

Result on the web

<a href="https://jcs.biologists.org/content/133/22/jcs249938" target="_blank" >https://jcs.biologists.org/content/133/22/jcs249938</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/jcs.249938" target="_blank" >10.1242/jcs.249938</a>

Result language

angličtina

Original language name

Stable tug-of-war between kinesin-1 and cytoplasmic dynein upon different ATP and roadblock concentrations

Original language description

The maintenance of intracellular processes, like organelle transport and cell division, depend on bidirectional movement along microtubules. These processes typically require kinesin and dynein motor proteins, which move with opposite directionality. Because both types of motors are often simultaneously bound to the cargo, regulatory mechanisms are required to ensure controlled directional transport. Recently, it has been shown that parameters like mechanical motor activation, ATP concentration and roadblocks on the microtubule surface differentially influence the activity of kinesin and dynein motors in distinct manners. However, how these parameters affect bidirectional transport systems has not been studied. Here, we investigate the regulatory influence of these three parameters using in vitro gliding motility assays and stochastic simulations. We find that the number of active kinesin and dynein motors determines the transport direction and velocity, but that variations in ATP concentration and roadblock density have no significant effect. Thus, factors influencing the force balance between opposite motors appear to be important, whereas the detailed stepping kinetics and bypassing capabilities of the motors only have a small effect.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cell Science

ISSN

0021-9533

e-ISSN

—

Volume of the periodical

133

Issue of the periodical within the volume

22

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

jcs249938

UT code for WoS article

000595804600009

EID of the result in the Scopus database

2-s2.0-85097037961