A prostate-specific membrane antigen activated molecular rotor for real-time fluorescence imaging
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F21%3A00548111" target="_blank" >RIV/86652036:_____/21:00548111 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41467-021-25746-6" target="_blank" >https://www.nature.com/articles/s41467-021-25746-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-021-25746-6" target="_blank" >10.1038/s41467-021-25746-6</a>
Alternative languages
Result language
angličtina
Original language name
A prostate-specific membrane antigen activated molecular rotor for real-time fluorescence imaging
Original language description
Surgery is an efficient way to treat localized prostate cancer (PCa), however, it is challenging to demarcate rapidly and accurately the tumor boundary intraoperatively, as existing tumor detection methods are seldom performed in real-time. To overcome those limitations, we develop a fluorescent molecular rotor that specifically targets the prostate-specific membrane antigen (PSMA), an established marker for PCa. The probes have picomolar affinity (IC50 = 63-118 pM) for PSMA and generate virtually instantaneous onset of robust fluorescent signal proportional to the concentration of the PSMA-probe complex. In vitro and ex vivo experiments using PCa cell lines and clinical samples, respectively, indicate the utility of the probe for biomedical applications, including real-time monitoring of endocytosis and tumor staging. Experiments performed in a PCa xenograft model reveal suitability of the probe for imaging applications in vivo.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA18-04790S" target="_blank" >GA18-04790S: Engineered antibodies as platforms for cancer imaging and therapy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Communications
ISSN
2041-1723
e-ISSN
2041-1723
Volume of the periodical
12
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
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UT code for WoS article
000697264200005
EID of the result in the Scopus database
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