Developmental Changes in Peripherin-eGFP Expression in Spiral Ganglion Neurons
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F21%3A00548736" target="_blank" >RIV/86652036:_____/21:00548736 - isvavai.cz</a>
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fncel.2021.678113/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fncel.2021.678113/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fncel.2021.678113" target="_blank" >10.3389/fncel.2021.678113</a>
Alternative languages
Result language
angličtina
Original language name
Developmental Changes in Peripherin-eGFP Expression in Spiral Ganglion Neurons
Original language description
The two types of spiral ganglion neurons (SGNs), types I and II, innervate inner hair cells and outer hair cells, respectively, within the mammalian cochlea and send another process back to cochlear nuclei in the hindbrain. Studying these two neuronal types has been made easier with the identification of unique molecular markers. One of these markers, peripherin, was shown using antibodies to be present in all SGNs initially but becomes specific to type II SGNs during maturation. We used mice with fluorescently labeled peripherin (Prph-eGFP) to examine peripherin expression in SGNs during development and in aged mice. Using these mice, we confirm the initial expression of Prph-eGFP in both types I and II neurons and eventual restriction to only type II perikarya shortly after birth. However, while Prph-eGFP is uniquely expressed within type II cell bodies by P8, both types I and II peripheral and central processes continue to express Prph-eGFP for some time before becoming downregulated. Only at P30 was there selective type II Prph-eGFP expression in central but not peripheral processes. By 9 months, only the type II cell bodies and more distal central processes retain Prph-eGFP expression. Our results show that Prph-eGFP is a reliable marker for type II SGN cell bodies beyond P8, however, it is not generally a suitable marker for type II processes, except for central processes beyond P30. How the changes in Prph-eGFP expression relate to subsequent protein expression remains to be explored.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
<a href="/en/project/GA20-06927S" target="_blank" >GA20-06927S: The role of NEUROD1 and ISL1 in neuronal development of the inner ear</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Cellular Neuroscience
ISSN
1662-5102
e-ISSN
1662-5102
Volume of the periodical
15
Issue of the periodical within the volume
JUN 15 2021
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
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UT code for WoS article
000667688500001
EID of the result in the Scopus database
2-s2.0-85109015187