alpha V Integrin Expression and Localization in Male Germ Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F21%3A00550583" target="_blank" >RIV/86652036:_____/21:00550583 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/21:88439
Result on the web
<a href="https://www.mdpi.com/1422-0067/22/17/9525" target="_blank" >https://www.mdpi.com/1422-0067/22/17/9525</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms22179525" target="_blank" >10.3390/ijms22179525</a>
Alternative languages
Result language
angličtina
Original language name
alpha V Integrin Expression and Localization in Male Germ Cells
Original language description
Integrins are transmembrane receptors that facilitate cell adhesion and cell-extracellular matrix communication. They are involved in the sperm maturation including capacitation and gamete interaction, resulting in successful fertilization. alpha V integrin belongs to the integrin glycoprotein superfamily, and it is indispensable for physiological spermiogenesis and testosterone production. We targeted the gene and protein expression of the alpha V integrin subunit and described its membrane localization in sperm. Firstly, in mouse, we traced alpha V integrin gene expression during spermatogenesis in testicular fraction separated by elutriation, and we detected gene activity in spermatogonia, spermatocytes, and round spermatids. Secondly, we specified alpha V integrin membrane localization in acrosome-intact and acrosome-reacted sperm and compared its pattern between mouse, pig, and human. Using immunodetection and structured illumination microscopy (SIM), the alpha V integrin localization was confined to the plasma membrane covering the acrosomal cap area and also to the inner acrosomal membrane of acrosome-intact sperm of all selected species. During the acrosome reaction, which was induced on capacitated sperm, the alpha V integrin relocated and was detected over the whole sperm head. Knowledge of the integrin pattern in mature sperm prepares the ground for further investigation into the pathologies and related fertility issues in human medicine and veterinary science.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Volume of the periodical
22
Issue of the periodical within the volume
17
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
9525
UT code for WoS article
000695595600001
EID of the result in the Scopus database
2-s2.0-85114097985