Kinetic Study of 17 alpha-Estradiol Mechanism during Rat Sperm Capacitation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F22%3A00559402" target="_blank" >RIV/86652036:_____/22:00559402 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1420-3049/27/13/4092" target="_blank" >https://www.mdpi.com/1420-3049/27/13/4092</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules27134092" target="_blank" >10.3390/molecules27134092</a>
Alternative languages
Result language
angličtina
Original language name
Kinetic Study of 17 alpha-Estradiol Mechanism during Rat Sperm Capacitation
Original language description
17 alpha-Estradiol (alpha E2) is a natural diastereoisomer of 17 beta-estradiol (E2). It is well known that alpha E2 can bind to estrogen receptors. However, its biological activity is less than that of E2 and is species and tissue specific. The goal of our study was to propose the mechanism of alpha E2 hormonal response in rat sperm during their capacitation in vitro and compare it with a previously studied mouse model. Concentration changes in externally added alpha E2 during capacitation of rat sperm were monitored by the high-performance liquid chromatographic method with tandem mass spectrometric detection (HPLC-MS/MS). The calculated values of relative concentrations B-t were subjected to kinetic analysis. The findings indicated that alpha E2 in rat sperm did not trigger autocatalytic reaction, in contrast to the mouse sperm, and that the initiation of the hormone penetration through the sperm plasma membrane was substantially faster in rats.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecules
ISSN
1420-3049
e-ISSN
1420-3049
Volume of the periodical
27
Issue of the periodical within the volume
13
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
4092
UT code for WoS article
000824750800001
EID of the result in the Scopus database
2-s2.0-85133232087