Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F23%3A00568516" target="_blank" >RIV/86652036:_____/23:00568516 - isvavai.cz</a>
Alternative codes found
RIV/CZ______:_____/23:N0000094 RIV/CZ______:_____/23:N0000033 RIV/00216208:11310/23:10476903
Result on the web
<a href="https://www.mdpi.com/2218-273X/13/1/161" target="_blank" >https://www.mdpi.com/2218-273X/13/1/161</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/biom13010161" target="_blank" >10.3390/biom13010161</a>
Alternative languages
Result language
angličtina
Original language name
Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies
Original language description
Time-resolved femtosecond-stimulated Raman spectroscopy (FSRS) provides valuable information on the structural dynamics of biomolecules. However, FSRS has been applied mainly up to the nanoseconds regime and above 700 cm(-1), which covers only part of the spectrum of biologically relevant time scales and Raman shifts. Here we report on a broadband (similar to 200-2200 cm(-1)) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump. The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222. The observed lifetimes and intermediate states (singlet, triplet, and adduct) are in agreement with previous time-resolved infrared spectroscopy experiments. Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm(-1). We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomolecules
ISSN
2218-273X
e-ISSN
2218-273X
Volume of the periodical
13
Issue of the periodical within the volume
1
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
161
UT code for WoS article
000914819600001
EID of the result in the Scopus database
2-s2.0-85146816730