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Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F23%3A00568516" target="_blank" >RIV/86652036:_____/23:00568516 - isvavai.cz</a>

  • Alternative codes found

    RIV/CZ______:_____/23:N0000094 RIV/CZ______:_____/23:N0000033 RIV/00216208:11310/23:10476903

  • Result on the web

    <a href="https://www.mdpi.com/2218-273X/13/1/161" target="_blank" >https://www.mdpi.com/2218-273X/13/1/161</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3390/biom13010161" target="_blank" >10.3390/biom13010161</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Sub-Millisecond Photoinduced Dynamics of Free and EL222-Bound FMN by Stimulated Raman and Visible Absorption Spectroscopies

  • Original language description

    Time-resolved femtosecond-stimulated Raman spectroscopy (FSRS) provides valuable information on the structural dynamics of biomolecules. However, FSRS has been applied mainly up to the nanoseconds regime and above 700 cm(-1), which covers only part of the spectrum of biologically relevant time scales and Raman shifts. Here we report on a broadband (similar to 200-2200 cm(-1)) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump. The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222. The observed lifetimes and intermediate states (singlet, triplet, and adduct) are in agreement with previous time-resolved infrared spectroscopy experiments. Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm(-1). We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biomolecules

  • ISSN

    2218-273X

  • e-ISSN

    2218-273X

  • Volume of the periodical

    13

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    12

  • Pages from-to

    161

  • UT code for WoS article

    000914819600001

  • EID of the result in the Scopus database

    2-s2.0-85146816730