Modulatory effect of MG-132 proteasomal inhibition on boar sperm motility during in vitro capacitation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F23%3A00571543" target="_blank" >RIV/86652036:_____/23:00571543 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/23:96543 RIV/00216208:11310/23:10464241

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fvets.2023.1116891/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fvets.2023.1116891/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fvets.2023.1116891" target="_blank" >10.3389/fvets.2023.1116891</a>

Result language

angličtina

Original language name

Modulatory effect of MG-132 proteasomal inhibition on boar sperm motility during in vitro capacitation

Original language description

A series of biochemical and biophysical changes during sperm capacitation initiates various signaling pathways related to protein phosphorylation leading to sperm hyperactivation, simultaneously with the regulation of proteasomal activity responsible for protein degradation and turnover. Our study aimed to unveil the role of the proteasome in the regulation of boar sperm motility, hyperactivated status, tyrosine phosphorylation, and total protein ubiquitination. The proteolytic activity of the 20S proteasomal core was inhibited by MG-132 in concentrations of 10, 25, 50, and 100 mu M, and monitored parameters were analyzed every hour during 3 h of in vitro capacitation (IVC). Sperm motility and kinematic parameters were analyzed by Computer Assisted Sperm Analysis (CASA) during IVC, showing a significant, negative, dose-dependent effect of MG-132 on total and progressive sperm motility (TMOT, PMOT, respectively). Furthermore, proteasomal inhibition by 50 and 100 mu M MG-132 had a negative impact on velocity-based kinematic sperm parameters (VSL, VAP, and VCL). Parameters related to the progressivity of sperm movement (LIN, STR) and ALH were the most affected by the highest inhibitor concentration (100 mu M). Cluster analysis revealed that the strongest proteasome-inhibiting treatment had a significant effect (p <= 0.05) on the hyperactivated sperm subpopulation. The flow cytometric viability results proved that reduced TMOT and PMOT were not caused by disruption of the integrity of the plasma membrane. Neither the protein tyrosine phosphorylation profile changes nor the accumulation of protein ubiquitination was observed during the course of capacitation under proteasome inhibition. In conclusion, inhibition of the proteasome reduced the ability of spermatozoa to undergo hyperactivation, however, there was no significant effect on the level of protein tyrosine phosphorylation and accumulation of ubiquitinated proteins. These effects might be due to the presence of compensatory mechanisms or the alteration of various ubiquitin-proteasome system-regulated pathways.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40301 - Veterinary science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Veterinary Science

ISSN

2297-1769

e-ISSN

2297-1769

Volume of the periodical

10

Issue of the periodical within the volume

MAR 23 2023

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

1116891

UT code for WoS article

000963854400001

EID of the result in the Scopus database

2-s2.0-85152576284