Determining Potency of Inhibitors Targeting Histone Deacetylase 6 by Quantification of Acetylated Tubulin in Cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F23%3A00578923" target="_blank" >RIV/86652036:_____/23:00578923 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-2788-4_29" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-2788-4_29</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-2788-4_29" target="_blank" >10.1007/978-1-0716-2788-4_29</a>

Result language
angličtina
Original language name
Determining Potency of Inhibitors Targeting Histone Deacetylase 6 by Quantification of Acetylated Tubulin in Cells
Original language description
During the preclinical development of small molecule inhibitors, compounds or compound libraries are typically first screened using purified target enzymes in vitro to select candidates with high potency. In the later stages of the development, however, functional cell-based assays may provide biologically more relevant data. In this chapter, we describe a detailed protocol for determining the potency of inhibitors targeting human histone deacetylase 6 in complex cellular environments. Cells are first treated with a dilution series of tested compounds, cell lysates separated by SDS-PAGE, and electrotransferred to a blotting membrane. The inhibitor potency is then determined indirectly by quantifying the levels of acetylated tubulin as a surrogate readout.
Czech name
—
Czech description
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Project
<a href="/en/project/GA21-31806S" target="_blank" >GA21-31806S: Modulation of structural and functional properties of the HSP90 chaperone machinery by reversible acetylation</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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