A highly active S1-P1 nuclease from the opportunistic pathogen <i>Stenotrophomonas maltophilia</i> cleaves c-di-GMP
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F23%3A00583248" target="_blank" >RIV/86652036:_____/23:00583248 - isvavai.cz</a>
Result on the web
<a href="https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14683" target="_blank" >https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.14683</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/1873-3468.14683" target="_blank" >10.1002/1873-3468.14683</a>
Alternative languages
Result language
angličtina
Original language name
A highly active S1-P1 nuclease from the opportunistic pathogen <i>Stenotrophomonas maltophilia</i> cleaves c-di-GMP
Original language description
A number of multidrug-resistant bacterial pathogens code for S1-P1 nucleases with a poorly understood role. We have characterized a recombinant form of S1-P1 nuclease from Stenotrophomonas maltophilia, an opportunistic pathogen. S. maltophilia nuclease 1 (SmNuc1) acts predominantly as an RNase and is active in a wide range of temperatures and pH. It retains a notable level of activity towards RNA and ssDNA at pH 5 and 9 and about 10% of activity towards RNA at 10 degrees C. SmNuc1 with very high catalytic rates out-performs S1 nuclease from Aspergillus oryzae and other similar nucleases on all types of substrates. SmNuc1 degrades second messenger c-di-GMP, which has potential implications for its role in the pathogenicity of S. maltophilia.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/GA20-12109S" target="_blank" >GA20-12109S: The Unique Mycobacterial Transcription Machinery: Insights into its Composition, Structure & Functioning.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEBS Letters
ISSN
0014-5793
e-ISSN
1873-3468
Volume of the periodical
597
Issue of the periodical within the volume
16
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
2103-2118
UT code for WoS article
001014859300001
EID of the result in the Scopus database
2-s2.0-85162950845