FAM110A promotes mitotic spindle formation by linking microtubules with actin cytoskeleton
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F24%3A00598889" target="_blank" >RIV/86652036:_____/24:00598889 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/24:00598889
Result on the web
<a href="https://www.pnas.org/doi/10.1073/pnas.2321647121" target="_blank" >https://www.pnas.org/doi/10.1073/pnas.2321647121</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.2321647121" target="_blank" >10.1073/pnas.2321647121</a>
Alternative languages
Result language
angličtina
Original language name
FAM110A promotes mitotic spindle formation by linking microtubules with actin cytoskeleton
Original language description
Precise segregation of chromosomes during mitosis requires assembly of a bipolar mitotic spindle followed by correct attachment of microtubules to the kinetochores. This highly spatiotemporally organized process is controlled by various mitotic kinases and molecular motors. We have recently shown that Casein Kinase 1 (CK1) promotes timely progression through mitosis by phosphorylating FAM110A leading to its enrichment at spindle poles. However, the mechanism by which FAM110A exerts its function in mitosis is unknown. Using structure prediction and a set of deletion mutants, we mapped here the interaction of the N- and C- terminal domains of FAM110A with actin and tubulin, respectively. Next, we found that the FAM110A-Delta 40- 61 mutant deficient in actin binding failed to rescue defects in chromosomal alignment caused by depletion of endogenous FAM110A. Depletion of FAM110A impaired assembly of F- actin in the proximity of spindle poles and was rescued by expression of the wild- type FAM110A, but not the FAM110A-Delta 40- 61 mutant. Purified FAM110A promoted binding of F- actin to microtubules as well as bundling of actin filaments in vitro. Finally, we found that the inhibition of CK1 impaired spindle actin formation and delayed progression through mitosis. We propose that CK1 and FAM110A promote timely progression through mitosis by mediating the interaction between spindle microtubules and filamentous actin to ensure proper mitotic spindle formation.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
1091-6490
Volume of the periodical
121
Issue of the periodical within the volume
29
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
e2321647121
UT code for WoS article
001282943000004
EID of the result in the Scopus database
2-s2.0-85198975396