17α-Ethynylestradiol alters testicular epigenetic profiles and histone-to-protamine exchange in mice

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F24%3A00600799" target="_blank" >RIV/86652036:_____/24:00600799 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10488231

Result on the web

<a href="https://rbej.biomedcentral.com/articles/10.1186/s12958-024-01307-6" target="_blank" >https://rbej.biomedcentral.com/articles/10.1186/s12958-024-01307-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12958-024-01307-6" target="_blank" >10.1186/s12958-024-01307-6</a>

Result language

angličtina

Original language name

17α-Ethynylestradiol alters testicular epigenetic profiles and histone-to-protamine exchange in mice

Original language description

Spermatogenesis starts with the onset of puberty within the seminiferous epithelium of the testes. It is a complex process under intricate control of the endocrine system. Physiological regulations by steroid hormones in general and by estrogens in particular are due to their chemical nature prone to be disrupted by exogenous factors acting as endocrine disruptors (EDs). 17 alpha-Ethynylestradiol (EE2) is an environmental pollutant with a confirmed ED activity and a well-known effect on spermatogenesis and chromatin remodeling in haploid germ cells. The aim of our study was to assess possible effects of two doses (2.5ng/ml, 2.5 mu g/ml) of EE2 on both histone-to-protamine exchange and epigenetic profiles during spermatogenesis performing a multi/transgenerational study in mice. Our results demonstrated an impaired histone-to-protamine exchange with a significantly higher histone retention in sperm nuclei of exposed animals, when this process was accompanied by the changes of histone post-translational modifications (PTMs) abundancies with a prominent effect on H3K9Ac and partial changes in protamine 1 promoter methylation status. Furthermore, individual changes in molecular phenotypes were partially transmitted to subsequent generations, when no direct trans-generational effect was observed. Finally, the uncovered specific localization of the histone retention in sperm nuclei and their specific PTMs profile after EE2 exposure may indicate an estrogenic effect on sperm motility and early embryonic development via epigenetic mechanisms.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Reproductive Biology and Endocrinology

ISSN

1477-7827

e-ISSN

1477-7827

Volume of the periodical

22

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

135

UT code for WoS article

001346667500001

EID of the result in the Scopus database

2-s2.0-85208483872